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Ellenberger and co-workers (eight) had intuited accurately such a motion from the OB domain after they solved the crystal structure with the much bigger human DNA ligase I (HuLig1; 919 amino acids) certain to an adenylylated nick. Their landmark structure was the primary to document the circumferential envelopment from the DNA duplex by a ligase clamp and also the resulting bending and distortion on the duplex that drive the base pairs about the 3′-OH aspect of the nick into an RNA-similar to a helical conformation (8). Similar distortions have been observed while in the ChVLig·DNA elaborate (7).
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